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Ube1 Protein, recombinant, 10 μg
Description:
Ube1, recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
Recombinant fusion protein containing human Ube1 with an N-terminal His-tag, expressed in sf9 insect cells.
Applications:
Recombinant fusion protein containing human Ube1 with an N-terminal His-tag, expressed in sf9 insect cells. For use in Activity Assays.
Key Applications:
Activity Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
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UniProt Summary:
FUNCTION: SwissProt: P22314 # Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
SIZE: 1058 amino acids; 117849 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family.
MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
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Species:
Human
Molecular Weight:
121 kDa
Brand Family:
Upstate
Source:
sf9 insect cells
Presentation:
10 µg recombinant Ube1 in 20 µL of 50mM Tris-HCl, pH 7.5, 270mM Sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 0.2mM PMSF, and 1mM Benzamidine. Frozen solution.
Storage Conditions:
Stable for 2 years at -70°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
UBE1
MGC4781
A1S9T
A1S9
A1ST
UBE1X
GXP1
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Product Name:
Ube1, recombinant
Alternate Names:
ubiquitin-activating enzyme E1
Quality Assurance:
Routinely evaluated by ubiquitin assay
UniProt Number:
P22314
Entrez Gene Number:
NM_003334.2
NM_153280.1
Purity:
75% by SDS PAGE and Coomassie blue staining.
Purification Method:
ProBond Resin chromatography |