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Anti-Chromobox protein homolog 7 (Cbx7)
Species Reactivity Key Applications Host Format Antibody Type
M WB, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Cbx7 Antibody
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Specificity:
This antibody recognizes Cbx7 at the C-terminus.
Molecular Weight:
~21 kDa observed. A non-specific band at ~ 44 kDa and may be observed in some lysates.
Epitope:
C-terminus
Immunogen:
GST-tagged recombinant protein corresponding to the C-terminus of mouse Cbx7.
Background Information:
Chromobox protein homologue 7 (Cbx7) is a member of the mammalian chromobox family of proteins. As a component of the Polycomb repressive complex 1 (PRC1), Cbx7, is involved in the maintenance and prolongation of cultured epithelial cell lifespan, and has been shown to function independently of BMI-1 in the repression of INK4a/ARF, a known tumor suppressor locus. Cbx7 is also able to regulate cellular lifespan through maintaining CDKN2A repression. Studies have shown Cbx7 to be up-regulated in follicular lymphoma, suggesting potential as a human oncogene.
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Species Reactivity:
Mouse
Application Notes:
Western Blot (Snap i.d.®) Analysis: 2 µg/mL from a representative lot detected Cbx7 on 10 µg of human and mouse embryonic stem cell lysates.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
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Control:
Mouse embryonic stem cell lysate
Quality Assurance:
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Cbx7 on 10 µg of mouse embryonic stem cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q8VDS3
Entrez Gene Number:
NP_659060
Gene Symbol:
CBX7
Cbx7
Alternate Names:
chromobox homolog 7
chromobox protein homolog 7
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
UniProt Summary:
FUNCTION: Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility (By similarity). Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity.
SUBUNIT STRUCTURE: Component of the chromatin-associated polycomb complex (PcG). Interacts with RING2 (By similarity). Interacts with RING1, but not with BMI1, EED or EZH2.
SUBCELLULAR LOCATION: Nucleus
DEVELOPMENTAL STAGE: In embryonic fibroblast cultured in vitro, expression gradually decreases with passage number and totally disappears in senescent cells.
SEQUENCE SIMILARITIES: Contains 1 chromo domain.
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Product Name:
Anti-Chromobox protein homolog 7 (Cbx7)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
1 |