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TurboFect™ Protein Transfection Reagent
125 μl#R1411
(for 125 transfections in a 24-well plate)
Lot: __
Expiry Date: __
Supplied with:
125 μl Enhancer Solution 10 μg (0.5 mg/ml) Fluorescein-labeled Rabbit IgG Control
Storage
Store TurboFect™ Protein Transfection Reagent and
Enhancer Solution at 4°C.
Store Fluorescein-labeled Rabbit IgG Control at -20°C in
the dark.
Ship at -20°C.
Description TurboFect™ Protein Transfection Reagent* is a sterile solution of a proprietary cationic polymer in water. In combination with the included Enhancer Solution*, the reagent efficiently delivers functionally active proteins, antibodies and peptides into the cytoplasm of cells. Proteins of different sizes and net charge (different pI) can be delivered into various cell types, including primary, adherent and suspension cells. The reagent can be used in the presence or absence of serum. Fluorescein-labeled Rabbit IgG Control is supplied as a control protein to determine transfection efficiency.
General Considerations
Cell Density The recommended confluency for adherent cells at the day of transfection is 70-90%. Suspension cells should be plated at an optimal density ensuring logarithmic growth at the time of transfection.
Incubation Time The shortest recommended incubation time for the transfection reagent/protein complexes with cells is 2 hours.
Transfection Reagent/Protein Ratio The volume of transfection reagent used depends on the amount of protein and type of cell to be transfected. The ratios presented in the protocols below are generalized values and can be further optimized for best results.
Transfection in the Presence of Serum Transfection in serum-free medium is recommended for best results. Transfection can be performed in the presence of serum; however, transfection efficiency may be reduced by up to 50%. |