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Pierce Fast Semi-Dry Blotter
For high-quality, high-throughput semi-dry transfer from protein gels in less than 10 minutes.
The Thermo Scientific Pierce Fast Semi-Dry Blotter is an electrophoresis apparatus for rapid and complete gel-to-membrane protein transfer following SDS-PAGE for PVDF or nitrocellulose Western blotting.
The Pierce Fast Semi-Dry Blotter is a durable and versatile electrophoretic transfer device that is large enough to process four separate protein mini gels at a time. Unlike other blotting systems, this blotter does not require costly consumable plates or sponges. The Fast Semi-Dry Blotter is compatible with traditional semi-dry transfer buffers and protocols, including for nucleic acid methods. The gel blotter provides exceptionally rapid protein transfer (less than 10 minutes) when it is used together with the Pierce Fast Semi-Dry Transfer Buffer (Part No. 35035).
Highlights:
Efficient protein blotting – evenly and effectively transfers protein from polyacrylamide gels to nitrocellulose or PVDF membranes
Fast electrophoretic transfer – blots protein onto membrane in 7 to 10 minutes when used with the methanol-free Pierce Semi-Dry Transfer Buffer, Part No. 35035
Sensitive yet economical – provides same transfer efficiency as other semi-dry or wet-transfer units but requires no special or costly consumables
Robust, high-capacity – large (20 x 20cm) metal plate electrodes provide a uniform electric field to ensure high-quality transfer for up to 4 mini gels at one time
Versatile – use for transfer from homemade or pre-cast polyacrylamide gels; with the Pierce Semi-Dry Transfer Buffer or conventional buffers; or any membrane type for Western blotting or nucleic acid detection
Product Details:
When used together, the Pierce Fast Semi-Dry Blotter and Pierce Fast Semi-Dry Transfer Buffer (Part No. 35035) provide a complete system for rapid, 10-minute protein transfer. The Transfer Buffer is supplied as a 10X concentrate for easy dilution with water before use, and the procedure does not require methanol.
The major advantages of fast semi-dry blotting are reduced buffer consumption, uniform electric field, high transfer efficiency, shorter transfer times and higher throughput (up to 4 mini gels at once). Nevertheless, the Pierce Fast Semi-Dry Blotter also can be used for conventional semi-dry transfer using traditional buffers and Western blotting protocols.
Transfer efficiency of the Thermo Scientific Pierce Fast Transfer System is comparable to existing methods. A549 whole cell lysates were prepared for SDS-PAGE and loaded onto a 4-12% Bis-Tris gel (1.0mm x 10 well) using the following protein amounts: 16, 8, 4 and 2µg. After electrophoresis, gels were transferred to nitrocellulose membrane using either the Pierce Fast Semi-Dry Transfer System or the Invitrogen iBlot* Dry Blotting System. Resulting membranes were probed for PRKDC, EGFR, PLK1, CDC2 and Cyclophilin B using the Pierce Fast Western Blotting Kit.
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