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Pierce Rapid ELISA Mouse mAB Isotyping Kit/快速ELISA小鼠抗体试剂盒
Pierce Rapid ELISA Mouse Antibody Isotyping Kit
Trouble-free determination of mouse antibody class and subclass identity for single or multiple samples.
The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.
This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.
Highlights:
Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
Specific – characterize antibodies for six different subclasses and two different light-chain types
Sensitive – accurate characterization with samples containing at least 3ng/mL of test antibody
Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
Cost effective – cost per assay is less than with single-use assay strips or cassettes
No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
Complete and easy to use – includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions
Description
Pierce Rapid ELISA Mouse mAb Isotyping Kit
Formulation: Multi-component kit
Sufficient For: 60 isotype determinations (8 wells per test sample)
Kit Contents:
Pre-coated 96-well Isotyping Plates, 5 plates
Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30mL
TMB Substrate, 55mL
Stop Solution, 55mL
30X Wash Buffer, 200mL
TBS Buffer Packs (each makes 500mL), 2 packs |