Pierce Fast Semi-Dry Transfer Buffer, 10X Complete, 10-minute protein transfer with semi-dry blotters for Western blotting. Write the first review Share this product Thermo Scientific Pierce Fast Semi-Dry Transfer Buffer, 10X, is a space-saving stock solution for preparing the methanol-free semi-dry transfer buffer to accomplish rapid (10-minute) Western blot transfer with semi-dry blotters. This Semi-Dry Transfer Buffer is designed to accelerate the transfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes in 7 to 10 minutes compared with the traditional 45-minute transfer. The transfer buffer was optimized for use with the Thermo Scientific Pierce Fast Semi-Dry Blotter (Part No. 88217) but is also compatible with other semi-dry transfer units. The formulation does not require methanol and is supplied as a 10X concentrate for easy dilution with water before use. Highlights: Optimized – formulated for accelerated and complete protein transfer with semi-dry blotters Non-hazardous – no methanol required, eliminating hazardous waste disposal Compatible – use with protein gels from all major types of SDS-PAGE (Tris-HEPES, Tris-glycine, Bis-Tris and Tris-HCl) Flexible – use with any semi-dry transfer unit; use with nitrocellulose or PVDF membranes Easy to use – simply dilute the 10X concentrated formula in ultrapure water; no need to add methanol or other components Product Details: For additional information, performance characteristics and features of the Pierce Fast Semi-Dry Transfer Buffer, please visit the page for the Pierce Fast Semi-Dry Blotter, Part No. 88217. |