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NBT/BCIP Stock Solution
Application
Colorimetric AP substrate for:
•Southern blot
•Western blot
•Immunohisto/cytochemistry
Product Description
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Molecular weights: NBT: Mr = 817.7, BCIP toluidine salt: Mr = 433.6
Reaction product:
Color: blue
Solubility in water: insoluble
Evaluation: visually
Contents
Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v) |