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Phenyl Sepharose CL-4B
Ligand: Phenyl
Average Particle Size: 90 μm
Matrix : 4 % Cross-linked agarose
Particle Size: 45 μm-165 μm
Ligand Concentration: 40 μlmole/ml drained medium
Binding Capacity/ml Chromatography Medium: HSA (Mr 68 000) 15-20 mg/ml drained medium, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained medium
Flow Velocity: <150 cm/h
Storage Conditions: 4 to 30°C, 20% Ethanol or 0.1 M NaOH
Chemical Stability: Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride.
Avoid Using: Oxidizing materials
Sanitization Conditions 0.5–1 M NaOH for at least 5 hours
Autoclavable: 121°C for 30 min |