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CM Sepharose™CL-6B is a weak cation
exchanger with excellent flow properties and
high capacity for proteins of all pI values. The
ion exchange group is a carboxy methyl group
which remains charged and maintains
consistently high capacity over the entire
working range, pH 6–12.
Type of ion exchanger: Weak cation
Total ionic capacity: 0.10–0.14 mmol H/ml medium
Available capacity*: IgG (MW 160 000) 9.5 mg/ml Bovine
Bovine COHb (MW 69 000) 75 mg/ml
Ribonuclease (MW 13 700) 120 mg/ml
Bead structure: 6% cross-linked agarose
Bead size range: 45–165 µm
Mean particle size: 90 µm
Max. linear flow rate**: 150 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
pH working range: 6–12
pH stability***
long term: 3–12
cleaning-in-place: 2–14
Chemical stability: All commonly used aqueous buffers, 1.0 M acetic acid,
1.0 M NaOH, 8 M Urea, 8 M guanidine hydrochloride,
ethanol, methanol etc.
Physical stability: Negligible volume variations due to changes in pH or ionic
strength
Autoclavable: In 0.1 M sodium acetate at 121 °C for 30 min |