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QAE Sephadex A-25
BioProcess Medium: No
Ligand: Diethyl-(2-hydroxy-propyl)aminoethyl
Ion Exchanger Type: Strong anion
Matrix: Cross-linked dextran
Partical Size Dry: 40 µm-120 µm
Binding Capacity/ml Chromatography Medium: thyroglobulin (Mr 669 000)- 1.5 mg/ml drained medium), HSA (Mr 68 000)- 10 mg/ml drained medium), α-lactalbumin (Mr 14 300)- 110 mg/ml drained medium)
Ionic Capacity: 2.6 mmol/g-3.4 mmol/g
pH Stability Working Range: 2-12
pH Stability Cleaning-in-Place (CIP): 2-13
Storage Conditions: 4 to 30°C, dry media. Used media 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Chemical Stability: Stable to all commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride.
Avoid Using: Oxidising agents and dextranase
Autoclavable: In 0.1 M sodium chloride at 121°C for 30 min. |