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Donkey Anti-Mouse IgG NL-637 Affinity Purified PAb, X Absorb|现货正品 Product Description This donkey anti-mouse IgG (H+L) antibody was purified by mouse IgG (H+L) affinity chromatography and absorbed against human, rabbit, bovine, goat, and Syrian hamster IgG to eliminate cross-reactivity. The purified antibody is then conjugated to fluorochrome NL637*. The spectral characteristics of NL637 are provided in Table 1. For comparison, the spectral characteristics of Allophycocyanin, Alexa Fluor 647, and Indodicarbocyanine (Cy5) are also included. Precaution Contains sodium azide as a preservative. Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large volumes of water during disposal. Dispose of azide containing liquids with caution and according to local regulations. Reagent Preparation Centrifuge the antibody solution in a microfuge before use. For most applications, a dilution of 1:200 is recommended. Optimal dilutions should be determined by each laboratory for each application. Intended Use For use as a secondary developing reagent in immunofluorescence assays including immunohistochemistry, immunocytochemistry, flow cytometry, and other fluorescent immunoassays with unlabeled primary antibodies of mouse origin. Storage Store the reagent at 2°- 8° C in the dark. Do not freeze. Under these conditions, the product is stable for at least 6 months from the date of receipt. |