SPECIFICATIONS SDSPAGE 3035 kDa, reducing conditions Activity Measured by its ability to inhibit BMP4induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.06-0.3 µg/mL in the presence of 75 ng/mL of rhBMP4. Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method. Purity >90%, by SDSPAGE under reducing conditions and visualized by silver stain. Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. See Certificate of Analysis for details.PREPARATION AND STORAGE Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
