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Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
Product Overview
Assay Kit Name:
Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
Kit Summary:
• Detection method - Absorbance (450 nm)
• Applications - Measurement of pyruvate dehydrogenase activity in various tissues/cells
- Analysis of cell signaling pathway
Sample Type:
• Animal tissues: heart, liver, muscle, etc.
• Purified mitochondria
• Cell culture: Adherent or suspension cells
Features & Benefits:
• Simple, rapid & convenient
• can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples
Kit Components:
• PDH Assay Buffer
• PDH Substrate (Lyophilized)
• PDH Developer (Lyophilized)
• NADH Standard (Lyophilized)
• PDH Positive Control
Description:
Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress. BioVision’s PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |