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Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit
Product Overview
Assay Kit Name:
Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit
Kit Summary:
• Detection method - Absorbance (450 nm)
• Applications - Measurement of α-Ketoglutarate dehydrogenase activity in various tissues/cells
• Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
Sample Type:
• Animal tissues: liver, heart, muscle, etc.
• Purified mitochondria
• Cell culture: Adherent or suspension cells
Features & Benefits:
• Simple, rapid & convenient
• can measure α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples
Kit Components:
• KGDH Assay Buffer
• KGDH Substrate (Lyophilized)
• KGDH Developer (Lyophilized)
• NADH Standard (Lyophilized)
• KGDH Positive Control
Description:
α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. BioVision’s α-KGDH assay kit provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |