MMP-3, PROENZYME, HUMAN

 MMP-3, Proenzyme, Human, Recombinant
Recombinant, human pro-MMP-3 purified from cell culture supernatant. May be used as a positive control or standard for zymographic analysis, or substrate assay. Requires activation for immunoblotting, prior to use. M.W. 57000/58000.
产品信息
Form  Lyophilized
Formulation  Lyophilized from 100 mM NaCl, 50 mM HEPES, pH 7.3.
Molar mass  57000
Applications 
Immunoblotting (Western Blotting)
Substrate Cleavage Assay
Zymography
Avoid freeze/thaw  是的
Activity  The activity of proenzyme MMP 3 was measured by substrate cleavage assay using 0.5 mM thiopeptiolide (Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-Oet) as a substrate. The activity was also assessed by degradation of a peptide substrate (DNP-PYAYWMR) using activated MMP-3 as measured by HPLC.
Purity  ≥95% by SDS-PAGE
Solubility  H2O
EC number  3.4.24.17
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling