K-LISA CHECKPOINT ACTIVITY KIT

K-LISA™ Checkpoint Activity Kit
K-LISA™ Chk1/Chk2 Activity Kit
This product has been discontinued.
The K-LISA™ Checkpoint Activity Kit is a rapid, sensitive, ELISA-based activity assay suitable for measuring the kinase activity of purified or partially purified Chk1 and Chk2 preparations, in vitro Chk1 and Chk2 inhibitor screening, and for assessing the regulation of Chk1 and Chk2 in cell signaling. The assay utilizes a biotinylated peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) that is phosphorylated on the third serine by Chk1 and Chk2. Biotinylated Chk substrate and sample containing Chk1 or Chk2 are incubated in the presence of ATP in wells of the streptavidin-coated 96-well plate, which allows for phosphorylation and substrate capture in a single step.
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
产品信息
Format  96-well plate
Form  96 Tests
Detection method  Colorimetric
Avoid freeze/thaw  是的
Sensitivity  25 pg (0.018 mU) purified Chk1
Assay range  25-725 µU (Chk1) and 260 µU - 26 mU (Chk2)
Assay time  3 h
Sample type  Cell lysates, tissue extracts, purified enzymes
Kit contains  Biotinylated Chk1 Substrate, Phosphoserine Detector Antibody, Streptavidin Coated 96-Well Plate Strips, HRP-Conjugated Secondary Antibody, Serine/Threonine Kinase Assay Buffer, ATP/MgCl2, Chk1 Inhibitor, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. Purified Chk1 is available separately (Cat. No. 220479).
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS