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Origami™ 2 Competent Cells
Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB (StrR, TetR)
相关产品
71345 Origami™ 2(DE3) Competent Cells
71346 Origami™ 2(DE3)pLysS Competent Cells
71431 Origami™ 2 Competent Cell Set*
产品信息
Components
0.4 ml 1 ml Component
• 2 × 0.2 ml 5 × 0.2 ml Origami™ 2 Competent Cells
• 2 × 2 ml 4 × 2 ml SOC Medium
• 2 ng 2 ng Test Plasmid
Guaranteed efficiency 2 × 106 cfu/µg
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |