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pIEx™-7 Ek/LIC Vector Kit
InsectDirect™ pIEx™-7 Ek/LIC Vector Kit
The pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1).
产品信息
Components
• 1 µg Ek/LIC Vector
• 25 units T4 DNA Polymerase, LIC-qualified
• 50 µl 25 mM EDTA
• 40 µl 25 mM dATP
• 8 µl Ek/LIC β-gal Control Insert
• 100 µl 100 mM DTT
• 50 µl 10X T4 DNA Polymerase Buffer
• 1.5 ml Nuclease-free Water
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |