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pIEx™-3 DNA
InsectDirect™ System- pIEx™-3 DNA
This product has been discontinued.
The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-3 contains the coding sequence for the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein. pIEx-3 also contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. Unique sites are shown on the circle map. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-3 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
For sequencing or PCR of recombinants in pIEx-3, refer to the table below to choose the appropriate primers.
Vector 5' Primer 3' Primer alt 5' Primer
pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling |