pBiEx™-1 DNA
InsectDirect™ System- pBiEx™-1 DNA
The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.
pBiEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. For sequencing or PCR of recombinants in pBiEx-1, refer to the table below to choose the appropriate primers.
The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Ccommercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Vector 5' Primer 3' Primer alt 5' Primer
pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer
NA
pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter Primer
pBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling |