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pSTBlue-1 AccepTor™ Vector Giga Kit
Featuring super-efficient competent cells for increased transformation efficiency
The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.
The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
相关产品
70594 Introductory pSTBlue-1 AccepTor™ Vector Kit
70595 pSTBlue-1 AccepTor™ Vector Kit
70596 pSTBlue-1 AccepTor™ Vector (linearized vector)
70597 Introductory pETBlue-1 AccepTor™ Vector Kit
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产品信息
Components
• 2 or 4 × 0.5 µg pSTBlue-1 AccepTor Vector
• 10 µl AccepTor™ Vector Positive Control Insert
• 2 or 4 × 55 µl Clonables™ 2X Ligation Premix
• 1.5 ml Nuclease-free Water
• 22 or 44 × 50 µl NovaBlue GigaSingles™ Competent Cells
• 4 or 7 × 2 ml SOC Medium
• 10 µl Test Plasmid
Features and benefits • No restriction enzymes or special primers
• Direct ligation of PCR product with vector
• Compatible with polymerases that leave single 3´-dA overhangs
• Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
• Simple protocol takes as little as 40 minutes from PCR product to plating transformants
Declaration This product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |