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pET NusA Fusion System 44
pET vectors plus host strains with induction control
This product has been discontinued.
pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET NusA Fusion System 44
The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag sequence for enhanced yields when purifying a target protein with His•Mag™ Agarose Beads from lysates generated with the BugBuster® HT Protein Extraction Reagent and PopCulture® Reagent (Novy 2002). N-terminal S•Tag and optional C-terminal HSV•Tag and His•Tag fusion sequences are also available for detection and purification of target proteins. The pET NusA Fusion System 44 is designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag™) protein. The solubility of many target proteins in the E. coli cytoplasm is increased when they are expressed NusA fusions. The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag® sequence for enhanced yields when purifying a target protein with His•Mag Agarose Beads from lysates generated with the BugBuster HT Reagent and PopCulture Reagent. N-terminal S•Tag™ and optional C-terminal HSV•Tag® and His•Tag fusion sequences are also available for versatile detection and purification of target proteins.
产品信息
Components
• 10 µg each pET-44 vector (pET-44a(+), pET-44b(+) and pET-44c(+))
• 0.2 ml Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
• 0.2 ml Induction control clone, glycerol stock
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling |