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PopCulture® GST•Mag™ Purification Kit
PopCulture extraction and GST•Tag™ fusion protein purification from E. coli cultures
This product has been discontinued.
The PopCulture® GST•Mag™ Purification Kit is designed for purification of GST•Tag™ fusion proteins directly from E. coli cultures without harvesting cells. The procedure combines PopCulture total culture extraction with magnetic affinity purification using GST•Mag Agarose Beads.
PopCulture Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for centrifugation. Use of GST•Mag Agarose Beads enables the entire procedure to be carried out in a single tube without using columns or centrifugation.
The PopCulture GST•Mag Purification Kit combines PopCulture Reagent, GST•Mag Agarose Beads, corresponding buffers and rLysozyme™ Solution. This kit enables processing of 40 × 3-ml cultures with yields up to 150 µg GST•Tag fusion protein per 3-ml culture, based on bead binding capacity. The kit is compatible with the Novagen Magnetight™ Separation Stand. For 96-well processing using PopCulture, see our various RoboPop™ Purification Kits.
产品信息
Components
• 15 ml PopCulture Reagent
• 3 × 1 ml GST•Mag Agarose Beads
• 2 × 100 ml 10X GST Bind/Wash Buffer
• 40 ml 10X Glutathione Reconstitution Buffer
• 1 g Glutathione, Reduced
• 300 KU rLysozyme Solution
• 1 ml rLysozyme Dilution Buffer
Features and benefits
Binding capacity of up to 2 mg/ml
Glutathione-derivatized magnetic agarose form with 3 µm average bead size
No need to separate cells from culture media: use 25 µl settled beads per milliliter culture (50 µl 50% v/v suspension)
No need to mechanically disrupt cells
No need to clarify cell extracts prior to purification
Direct affinity adsorption of target proteins to resin from the total culture extract
Ability to rapidly perform the entire cell growth and purification process in a single tube or well
Declaration This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
商店和运输信息
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS |