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RLYSOZYME SOLUTION

产品编号: 71110-5     查看说明书
产品名称: RLYSOZYME SOLUTION  .0   订购此产品 
供应商: Merck
规格: ***
目录价: 3587
库存状态: 3周到货
CAS编号:
应用范围: 生化实验
种属来源:
相关信息:

rLysozyme™ Solution
Stabilized recombinant lysozyme
rLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration r
ange of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
产品信息
Avoid freeze/thaw  是的
Unit definition  One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard HandlingStabilized recombinant lysozyme
rLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration r
ange of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
产品信息
Avoid freeze/thaw  是的
Unit definition  One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

保存条件: -20°C
说明书地址: 点击查看详细
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