|
pET-41 Ek/LIC Vector Kit
Directional PCR cloning into the most powerful E. coli expression vectors
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
相关产品
69076 pET-32 Ek/LIC Vector Kit
69077 pET-30 Ek/LIC Vector Kit
70072 pET-32 Xa/LIC Vector Kit
70073 pET-30 Xa/LIC Vector Kit
显示所有
产品信息
Components
• 1 µg Ek/LIC Vector
• 10 µl Ek/LIC Control Insert
• 25 U T4 DNA Polymerase, LIC-qualified
• 50 µl 10X T4 DNA Polymerase Buffer
• 100 µl 100 mM DTT
• 50 µl 25 mM EDTA
• 40 µl 25 mM dATP
• 1.5 ml Nuclease-free Water
• 22 × 50 µl NovaBlue GigaSingles™ Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 5 × 2 ml SOC Medium
• 10 µl Test Plasmid
Vector family pET
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |