HT96™ NovaBlue Competent Cells
The HT96™ NovaBlue Competent Cells are designed for high-throughput transformation. The cells are predispensed in 20-µl volumes in a sturdy 96-well polypropylene plate, which is compatible with a variety of thermal cyclers and water baths. Wells are individually sealed and have raised rims to prevent cross-contamination. Seals can be easily pierced with standard pipet tips or removed for access. Strips of caps are also provided for reliable sealing during manipulation and storage. Groups of 24 wells can be easily split from the plate for processing smaller numbers of samples.
HT96 NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host because it has high transformation efficiency, blue/white screening capability (with appropriate plasmids), and mutations in recA and endA, which result in high yields of high-quality plasmid DNA.
Genotype:
endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
产品信息
Avoid freeze/thaw 是的
Components
1 plate 4 plates
• 1 plate 4 plates HT96 NovaBlue Competent Cells
• 1 × 14 ml 4 × 14 ml SOC Medium
• 1 × 10 µl 2 × 10 µl Test Plasmid
• 1 pkg 4 pkg 8-Cap Strips
• 1 4 Reagent Reservoirs
Features and benefits • High-throughput, 96-well format
Genotype endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
Guaranteed efficiency > 1 × 108 cfu/µg
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS |