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Origami™ B Competent Cell Set
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™ and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotypes:
Origami B
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522:: Tn10 trxB (KanR, TetR)
Origami B(DE3)
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (KanR, TetR)
Origami B(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB pLysS (CamR, KanR, TetR)
相关产品
70836 Origami™ B Competent Cells
70837 Origami™ B(DE3) Competent Cells
70839 Origami™ B(DE3)pLysS Competent Cells
产品信息
Components
• 2 x 0.2 ml Origami B Competent Cells
• 2 x 0.2 ml Origami B(DE3) Competent Cells
• 2 x 0.2 ml Origami B(DE3)pLysS Competent Cells
• 4 x 2 ml SOC Medium
• 2 ng Test Plasmid
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |