pBAC™-3 DNA pBAC™-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer. 相关产品 70003 pBAC™-1 DNA 70004 pBAC™-2cp DNA 70049 pBACgus-2cp DNA 70054 pBAC™gus-1 DNA 显示所有 产品信息 Avoid freeze/thaw 是的 商店和运输信息 Storage -20°C Ship Shipped with Blue Ice or with Dry Ice Standard Handling