Single dA™ Tailing Kit Addition of single 3′-dA overhangs for cloning into AccepTor™ Vectors This product has been discontinued. The Single dA™ Tailing Kit is used to prepare DNA with any type of end for cloning into the Novagen AccepTor™ Vectors. The kit provides the reagents needed to blunt DNA ends and add a single 3′-dA residue. This treatment creates vector-compatible overhangs and effectively prevents insert-to-insert ligation, thus eliminating tandem inserts. The positive control included in the kit is the blunt-ended HSV•Tag® 36-mer that encodes the 11-aa peptide recognized by the HSV•Tag Monoclonal Antibody. Applications Treatment of PCR products of proofreading DNA polymerases for cloning into AccepTor Vectors (alternative to blunt-end cloning) Treatment of randomly cut or sheared DNA to be cloned into dU- or dT-tailed vectors 产品信息 Avoid freeze/thaw 是的 Components • 25 U T4 DNA Polymerase • 50 µl 10X Flush Buffer • 50 µl 10X dNTP Mix • 25 µl 100 mM DTT • 25 U Tth DNA Polymerase • 200 µl 10X dA Tailing Buffer • 500 ng Positive Control HSV•Tag® 36mer 商店和运输信息 Storage -20°C Ship Shipped with Blue Ice or with Dry Ice Multiple Toxicity Values, refer to MSDS