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β-Secretase Substrate IV, Fluorogenic
FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
产品信息
Peptide Sequence H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Form Red lyophilized solid
Formulation Supplied as a trifluoroacetate salt.
Molar mass 2005.2
Protect from Light 是的
Packaged under inert gas 是的
Purity ≥95% by HPLC
Excitation max. ~350 nm
Emission max. ~490 nm
Solubility DMSO
Chemical formula C91H129N25O25S
商店和运输信息
Storage -20°C
Ship Ambient Temperature Only
Standard Handling |