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Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ for optimal activity.
产品信息
Form Liquid
Formulation In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Molar mass 117000
Avoid freeze/thaw 是的
Biological activity 1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
Purity ≥90% by SDS-PAGE
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |