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Procathepsin K, Human, Recombinant, E. coli
Recombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (Cat. No. 108975) (IC50 = 70 nM), E-64 (Cat. No. 324890) (IC50 = 5 nM), and cystatin (Cat. No. 324891 or 324896). Requires activation prior to use. If the activated enzyme is not used immediately, it is recommended to add methyl methanthiosulfonate (1 mM final concentration MMTS). Note: 1 mU = 1 milliunit.
产品信息
Form Liquid
Formulation In 500 mM NaCl, 25 mM Tris, pH 8.0.
Molar mass 35068
Avoid freeze/thaw 是的
Unit definition One unit is defined as the amount of enzyme that will hydrolyze 1 µmole benzyloxycarbonyl-phenylalanine-arginine-7-amido-4-methylcoumarin per min at 37°C, pH 5.5.
Activity ≥1000 mU/mg protein
Purity ≥95% by SDS-PAGE
EC number 3.4.22.38
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |