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Calmodulin, Biotinylated, Porcine Brain
Calmodulin (CaM) purified from porcine brain and biotinylated using NHS ester-activated biotin with an 8 atom spacer arm. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates several biological processes, such as inflammation, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for enzyme activation, CaM binding studies, and general assessment of CaM-dependent signaling pathways. Biotin/Protein Ratio: 0.5-1.0 as determined by HABA assay.
产品信息
Form Lyophilized
Formulation Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
Molar mass 17000
Avoid freeze/thaw 是的
Specific activity ≥ 35,000 U/mg protein
Unit definition One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca2+ in a 3 ml reaction volume at 30°C, pH 7.5.
Purity >98% by SDS-PAGE
Solubility H2O
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Standard Handling |