CALPAINSUBSTRATEIV,CELL-PERMEABLE,FLUORO

Calpain Substrate IV, Cell-Permeable, Fluorogenic
The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
产品信息
Peptide Sequence  (DABCYL)-Trp-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH2
Form  Lyophilized solid
Formulation  Supplied as a trifluoroacetate salt
Molar mass  2897.4
Protect from Light  是的
Hygrocopic  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Excitation max.  480 nm
Emission max.  320 nm
Solubility  DMSO
Chemical formula  C127H206N50O27S
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling