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Calpain Substrate III, Fluorogenic
A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
产品信息
Peptide Sequence DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
Form Red lyophilized solid
Formulation Supplied as a trifluoroacetate salt.
Molar mass 1676
Protect from Light 是的
Packaged under inert gas 是的
Purity ≥98% by HPLC
Excitation max. 320 nm
Emission max. 480 nm
Solubility DMSO or H2O
Chemical formula C80H114N20O18S
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Irritant |