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Complement C5b,6 Complex, Human
Native, human C5b,6 complement component complex. Activation of complement by either pathway results in the formation of C5-cleaving enzymes (C5 convertases) on target surfaces. The C5 convertase enzymes cleave the C5 α-chain at peptide bond 74, resulting in the formation of the C5a anaphylatoxin (M.W. 11,200) and the C5b fragment (M.W. 185,000). The nascent C5b fragment can combine with C6, C7, C8, and C9 to form the lytic C5b-9 membrane attack complex on target surfaces. When C5 activation occurs in the presence of C6 only, e.g. in C7 deficient serum, a stable C5b,6 complex is formed. In a process termed Reactive Lysis, the membrane attack complex can be assembled on target cell surfaces in the absence of any additional complement proteins by incubating target cells with the C5b,6 complex and purified C7, C8, and C9.
产品信息
Form Liquid
Formulation In 120 mM NaCl, 10 mM HEPES, pH 7.2.
Molar mass 312000
Avoid freeze/thaw 是的
Specific activity ≥2000 C5b,6H50 units/mg
Purity ≥95% by SDS-PAGE
Source Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |