ACE, HISTAG, HU., RECOM, NSO CEL

Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells
Peptidyl-Dipetidase A, ACE
Recombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
产品信息
Form  Liquid
Formulation  In 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl2, 40% glycerol, pH 7.5.
Molar mass  120000
Avoid freeze/thaw  是的
Specific activity  ≥1 unit/µg protein
Unit definition  One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
Purity  ≥95% by SDS-PAGE
Contaminants  Endotoxins: ≤1.0 EU/µg ACE
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling