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AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda
AMP Kinase (α2/β1/γ1)
Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
产品信息
Form Liquid
Formulation In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
Avoid freeze/thaw 是的
Specific activity ≥270 nmol/min/mg protein
Unit definition Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
Purity ≥75% by SDS-PAGE
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling |