|
For robust two-step RT-PCR of RNA up to 14 kb
Application
The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications:
•Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments.
•Generating cDNA libraries with large and full-length inserts.
•Cloning genes of interest.
The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions.
Contents
1.Transcriptor Reverse Transcriptase
2.Transcriptor RT Reaction Buffer, 5x concentrated
3.Protector RNase Inhibitor
4.dNTP Mix, PCR Grade
5.Anchored Oligo (dT)18 Primer
6.Random Hexamer Primer
7.Control RNA – total RNA fraction purified from the immortalized cell line K-562.
Note: Included only in the 50-reaction pack size
8.Control PBGD Primer Mix (forward and reverse primer)
Note: Included only in the 50-reaction pack size
9.Water, PCR Grade
|