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TeloTAGGG Telomerase PCR ELISA formerly Telomerase PCR ELISA
Application
The TeloTAGGG Telomerase PCR ELISA is designed for use in life-science research studies for the highly sensitive qualitative detection of telomerase activity in cell extracts from cell cultures and other biological samples. The TeloTAGGG Telomerase PCR ELISA utilizes a single-tube reaction mixture that simplifies the amplification reaction. The ELISA technique uses a biotinylated primer to immobilize the TRAP reaction products within a streptavidin-coated microplate, and a specific DIG-labeled probe for detection.
Benefits
•Easy: One-step/one-tube ready-to-use reaction mix for elongation and amplification. Detection of up to 96 samples in an ELISA format without laborious gel separation.
•Reliable: Shows high correlation with standard TRAP assay
•Safe: Nonradioactive method that can be performed in any laboratory
•Complete: All components necessary for elongation, amplification, and detection are provided in the kit
Product Description
Assay time: Approx. 5 - 8 hours
Sample material: Cell or tissue extracts
Sensitivity: As sensitive as the radioactive (or isotopic) TRAP assay
Background Information
Telomeres are specialized DNA protein structures found at the end of eukaryotic chromosomes. Telomeric DNA is characterized by an array of tandemly repeated, G-rich DNA sequences (six nucleotides in length) that are highly conserved during evolution (human repeat sequence: TTAGGG). As DNA polymerase α is unable to replicate the very ends of linear DNA, every replication cycle leads to a progressive shortening of the telomeric ends in normal somatic cells. This phenomenon appears to be linked to the limited proliferative capacity of normal somatic cells of higher eukaryotes. Telomerase, a ribonucleoprotein, catalyzes the addition of TTAGGG repeats to the ends of vertebrate chromosomes, using a complementary sequence of its intrinsic RNA component as a template. Telomerase activity has been shown to be expressed in germ cells, as well as most cell lines and tumors. Escaping from the proliferative limitations of cellular senescence, telomerase reactivation may be a prerequisite of the development of malignant tumor cells from somatic cells.
Contents
1.Lysis Reagent
2.Reaction Mixture
3.Denaturation Reagent
4.Hybridization Buffer
5.Washing Buffer
6.Anti-DIG-POD
7.Conjugate Dilution Buffer
8.TMB Substrate Solution
9.Stop Reagent
10.Positive-control Cell Extract
11.Microplate
12.Cover Foils
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