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Taq DNA Polymerase, dNTPack 5 U/µl
Taq DNA Polymerase is a standard enzyme for the amplification of DNA fragments up to 3 kb by the polymerase chain reaction (PCR). It does not have proofreading or hot start features. Choose Roche Applied Science dNTPacks, convenient products that combine PCR grade nucleotides, thermostable enzymes and enzyme blends, and all associated components such as buffers and PCR-enhancing additives. Our PCR grade nucleotides are assayed for function in RT-PCR, ensuring optimal performance of all components. Each dNTPack contains the additive-free sodium salt nucleotides as a ready-to-use mix (10 mM of each dNTP).
Contents
1.Taq DNA Polymerase, 5 U/µl
2.PCR Buffer, 10x conc. with MgCl2
3.PCR Grade Nucleotide Mix
Application
Taq DNA Polymerase is the recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus BM, expressed in E. coli. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease activity.
•PCR
•RT-PCR
•Primer extension
Benefits
•Obtain reliable, reproducible results.
Roche Applied Science’s Taq DNA Polymerase exemplifies lot-to-lot consistency – tube-to-tube, experiment-to-experiment.
•Eliminate testing of each new lot.
Taq DNA Polymerase is rigorously tested, resulting in highest lot-to-lot consistency.
•Ensure optimal performance by using PCR-Grade Nucleotides.
Our high-purity nucleotides improve experimental consistency and sensitivity.
Product Description
The enzyme dNTPack comprises Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides. Achieve the most consistent results in simple, routine PCR by using Roche Applied Science’s Taq DNA Polymerase, which is held to our rigorous purity and quality standards. Before it arrives in your laboratory, every lot of our Taq DNA Polymerase must pass stringent tests for functionality and contaminating activities, ensuring reliable, consistent results with every lot. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (95°C). The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20°C) and temperatures around 75°C.
Volume activity: 5 U/µl |