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Protector RNase Inhibitor
Special Quality for Molecular Biology.
Benefits
Protector RNase Inhibitor inhibits a wide spectrum of RNases and protect your RNA samples from degradation (Figure 1 and 2). Use this versatile RNase inhibitor in combination with different enzymes and buffers between pH 5.0 – 9.0
•Rely on a thermostable RNase inhibitor, also when thermostable reverse transcriptases like Transcriptor Reverse Transcriptase are used.
•Benefit from a wide range of reaction conditions. Protector RNase Inhibitor is active at ph 5.0 to 9.0 and at temperatures between +25°C to +55°C (partial activity is still measureable at +60°C).
•Eliminate interference in different RNA analysis applications. Maintain performance even when adding at 16-fold higher than the standard concentration.
•Insist on a highly-purified preparation. Each batch is function tested using techniques like quantitative RT-PCR to ensure the absence of endonucleases, ribonucleases, or nicking activity.
Background Information
Protector RNase Inhibitor inactivates a wide spectrum of RNases, including RNase A, RNase B, and RNase T2 (Table 1). This thermostable enzyme is fully active during cDNA synthesis (e.g., with thermostable reverse transcriptases like Transcriptor Reverse Transcriptase, Table 2). Thus, Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems.
Quality
Each lot of Protector RNase Inhibitor is function tested with the Titan One Tube RT-PCR Kit* and the LightCycler DPD Kit*. Protector RNase Inhibitor is also tested for contaminating activities as described below.
Test buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.1 mM EDTA, 7 mM β-Mercaptoethanol; pH 7.5 (+37°C).
Absence of endonucleases: 1 μg Eco RI/Hind III*-fragments of lambda DNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no alteration in the banding pattern is stated under "Endo."
Absence of nicking activity: 1 μg supercoiled pBR322 DNA is incubated with Protector RNase Inhibitor in 50 μl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no relaxation of supercoiled DNA is stated under "Nick. Act."
Absence of ribonuclease (1): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under “RNase 1.”
Absence of ribonuclease (2): 5 μg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C, then 10 minutes at +65°C in a final volume of 50 μl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under “RNase 2.”
* available from Roche Apllied Science |