原装现货|Klenow Enzyme, Labeling Grade,

Klenow Enzyme Labeling grade, from Escherichia coli lysogenic NM 964
Application
Use this Labeling Grade of Klenow Enzyme for DNA labeling reactions and other common molecular biology procedures, including:
•3’-End labeling
•Random DNA labeling
•Filling in recessed (staggered) DNA ends (to create blunt-ended DNA)
•Elongation of oligonucleotides (e.g., during site-directed mutagenesis)
Product Description
Brief description: Klenow Enzyme (large fragment of E. coli DNA polymerase I) catalyzes the addition of mononucleotides from deoxynucleoside-5´-triphosphates to the 3´-hydroxyl ends of a primer. It retains the 5´ → 3´ polymerase and the 3´ → 5´ exonuclease activities of intact DNA polymerase I, but lacks the 5´ → 3´ exonuclease activity of the native enzyme.
Source: E. coli, lysogenic for NM 964
Molecular weight:  Mr = 75 kD
Background Information
Klenow Enzyme is formed by subtilisin treatment of DNA polymerase I.
Contents
Solution, 2 U/μl, in buffer, pH 7.0 (4oC)
Quality
Absence of contaminants: Labeling Grade Klenow Enzyme is tested on various substrates to ensure the absence of nonspecific endonucleases and exonucleases.