|
cOmplete ULTRA Tablets, Mini, EASYpack
Protease Inhibitor Cocktail Tablets supplied in foil blister packs.
Application
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche’s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).
Benefits
•Complete protection
The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.
•Convenient tablet format
Eliminate weighing small amounts, and instantly protect your proteins against a now even wider range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.
•Compatible with the new IMAC resin
For the purification of his-tagged proteins, Roche’s new EDTA-compatible cOmplete His-Tag Purification Resin is an ideal partner for this EDTA-containing tablet.
Product Description
cOmplete ULTRA Tablets contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. As this tablet contains EDTA, metalloproteases are also inhibited. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA, Mini Tablet is sufficient for a volume of 10 ml extraction solution.
Background Information
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. Metalloproteases, which are inhibited by EDTA, are fairly prevalent in, for example, bacteria.
Obtain convenient, reliable protection of your proteins against dephosphorylation using the new PhosSTOP Phosphatase Inhibitor Cocktail Tablets. You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
Contents
30 tablets individually packed in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
|