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Anti-Fluorescein-POD, Fab fragments from sheep. For the detection of fluorescein-labeled compounds. For life science research only. Not for use in diagnostic procedures. Fab fragments from polyclonal anti-fluorescein antibodies, conjugated to horseradish peroxidase. Contents Lyophilizate, stabilized Use Anti-Fluorescein-POD, Fab fragments for the detection of fluorescein-labeled compounds using: Dot blot ELISA Immunohisto/cytochemistry In situ hybridization Southern blot Western blot The reagent is an anti-fluorescein antibody, Fab fragments from sheep, conjugated with horse-radishperoxidase (POD). After immunization with fluorescein the sheep IgG was purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated with POD and stabilized in 60 mM Tris-Hepes-buffer, 0.4% bovine immunoglobulin (w/v), 0.2% Germall II (w/v), pH 7.2. Clone: polyclonal Ig class: sheep IgG, Fab fragments Specificity: The polyclonal antibodies react with free and bound fluorescein. Working concentration: Dot blot: 150 mU/ml ELISA: 50 - 150 mU/ml Immunohisto/cytochemistry: 250 - 500 mU/ml In situ hybridization: 1.5 - 7.5 U/ml Southern blot: 150 mU/ml Western blot: 500 - 1,000 mU/ml
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