描述Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion. All three pTracer™ vectors have the following features: •Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines•BGH polyA signal and transcription termination sequences to enhance mRNA stabilityThe pTracer™-SV40 vector offers: •SV40 promoter for high-level constitutive expression of your gene of interest•CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion•SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigenThe pTracer™-CMV2 vector offers: •CMV promoter for high-level constitutive 更看更多•expression of your gene of interest•EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion•Ampicillin resistance gene for selection in E. coliThe pTracer™-EF vectors offer: •EF-1α promoter for high-level constitutive expression of your gene of interest•CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion•C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody•C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody•Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag详细说明常用规格Promoter: SV40 Protein Tag or Fusion: Untagged Reporter: GFP (Cycle 3) Constitutive or Inducible System: Constitutive Delivery Method: Transfection Cloning Method: Restriction Enzyme ⁄ MCS Key Function: Reporter Assays Selection Agent (Eukaryotic): Zeocin™ Vector Type: pTracer Product Size: 20 µg Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. 内容和储存pTracer Kits contain 20 µg of the appropriate vector and 1 g of Zeocin (100 mg/ml).