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NFκB [pS536] Rabbit Polyclonal Antibody
Isotype
Rb IgG
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human NFkB that contains serine 536.
Specificity
Nuclear factor-κB is a family of transcription factors that consists of homo- and heterodimers of NFκB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino-terminus domain that includes dimeraization, nuclear localization, and DNA binding regions, as well as a carboxy-terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL-1β, and TNF-α as mediated by IκB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity.
Reactivity
Human NFkB. Mouse NFκB (91% homologous) has not been tested, but is expected to react.
Applications
Western blotting
Reactivity: Human
Validated Application: Western Blot
Host: Rabbit
SubClass (Isotype): IgG
Clonality: Polyclonal
Target Gene: NFkappaB (NFkB) [pS536]
Gene ID: 4790
Label or Dye: Unconjugated
Target Modification State: Phospho
Product Size: 100 µL
Protein Family: Transcription Factors
Research Category: Signal Transduction
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C). |