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Tfi DNA Polymerase
Tfi DNA Polymerase is a new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq, Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a component in a diagnostic application if qualified by the end user. With Tfi DNA Polymerase, you'll:
■Eliminate licensing fees and restrictions associated with Taq-based enzymes
■Save money-Tfi is half the price of Taq DNA Polymerase
■Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase
■No need to reoptimize - swap out Taq for Tfi
■Tfi is also available in special pack sizes and for OEM customers needing PCR kit manufacturing flexibility.
Polymerase: Tfi DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 4 kb or less
Product Size: 500 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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