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Biotin-14-CTP (Invitrogen™)
Biotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP (1). The biotinylated RNA can be used as a probe in membrane-based or in situ hybridizations (2). The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template).
Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC.
Label or Dye: Biotin
Product Size: 500 nmol
Concentration: 10 mM
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Biotin-14-CTP is supplied at 10 mM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C. |