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SuperScript® Choice System for cDNA Synthesis
The SuperScript® Choice System is designed for synthesis of double-stranded cDNA from a purified mRNA population. Advantages of the SuperScript® Choice system include:
■The advanced capabilities of SuperScript® II RT resulting in greater cDNA yields and increased full-length cDNAs (1,3)
■Choice of primers for first-strand synthesis (2)
■One-tube format improving cDNA yield (4,5)
■cDNA is size-fractionated using an efficient and convenient pre-packed column
■One reaction converts up to 5 µg of mRNA into size-fractionated, EcoR I-adapted cDNA, ready for ligation into any EcoR I-digested vector
Performance and Quality Testing: Yields of the first- and secondstrand cDNA synthesis reactions and percentage of full-length double-stranded cDNA are assessed using the control RNA.
Reverse Transcriptase: SuperScript® II
Downstream Application: cDNA Library Construction
Reaction Format: Separate Components
Sample Type (General): RNA
Product Size: 3 reactions
Final Product: cDNA (Second-Strand)
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature: 42° C
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